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3d imaris filaments  (Oxford Instruments)
99
Oxford Instruments 3d imaris filaments
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Imaris Filaments, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d printing filament owens corning xstrand gf30-pp  (Corning Life Sciences)
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Corning Life Sciences 3d printing filament owens corning xstrand gf30-pp
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Printing Filament Owens Corning Xstrand Gf30 Pp, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d printing filament owens corning xstrand gf30-pp/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
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3d printing filament basf innofil gf30  (BASF)
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BASF 3d printing filament basf innofil gf30
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Printing Filament Basf Innofil Gf30, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d printing filament basf innofil gf30/product/BASF
Average 90 stars, based on 1 article reviews
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3d fused filament printer up box plus  (Tiertime Corporation)
90
Tiertime Corporation 3d fused filament printer up box plus
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Fused Filament Printer Up Box Plus, supplied by Tiertime Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d fused filament printer up box plus/product/Tiertime Corporation
Average 90 stars, based on 1 article reviews
3d fused filament printer up box plus - by Bioz Stars, 2026-05
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3d imaris filament tracer  (Oxford Instruments)
99
Oxford Instruments 3d imaris filament tracer
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Imaris Filament Tracer, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d imaris filament tracer/product/Oxford Instruments
Average 99 stars, based on 1 article reviews
3d imaris filament tracer - by Bioz Stars, 2026-05
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3d printer with multiple filaments  (Markforged Inc)
90
Markforged Inc 3d printer with multiple filaments
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Printer With Multiple Filaments, supplied by Markforged Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d printer with multiple filaments/product/Markforged Inc
Average 90 stars, based on 1 article reviews
3d printer with multiple filaments - by Bioz Stars, 2026-05
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3d filament editor  (Amira Pharmaceuticals)
90
Amira Pharmaceuticals 3d filament editor
A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of <t>3D</t> morphometric reconstructions by <t>using</t> <t>Imaris</t> software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.
3d Filament Editor, supplied by Amira Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d filament editor/product/Amira Pharmaceuticals
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Image Search Results


A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of 3D morphometric reconstructions by using Imaris software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.

Journal: Communications Biology

Article Title: JMY powers dendritogenesis and is regulated by CaM revealing a general, critical principle in neuromorphogenesis

doi: 10.1038/s42003-025-08208-3

Figure Lengend Snippet: A Anti-MAP staining and GPF signals of primary hippocampal neurons transfected with plasmids driving the expression of either scrambled RNAi (Scr. RNAi) or JMY RNAi (RNAi#1) together with GFP as a marker or together with GFP and an RNAi#1-insensitive JMY using an IRES (RNAi#1/JMY*) at DIV4 and fixed 30 h thereafter. Asterisks mark transfected neurons. Bars, 30 μm. Lower panels show 2D representations of 3D morphometric reconstructions by using Imaris software. Note that viewing the images at high magnification also allows for seeing the Imaris-based markings of branch points (red) and terminal points (green). Quantitative determinations of specific defects in dendritic arborization caused by JMY deficiency by addressing dendritic branch points ( B ), dendritic terminal points ( C ), total dendritic tree length ( D ), as well as by conducting dendritic branch depth ( E ) and Sholl analyses ( F ). Data, mean ± SEM shown as bar/dot plots ( B–D ) and bar plot ( E , F ), respectively. Scr. RNAi, n = 44; RNAi#1, n = 46; RNAi#1/JMY*, n = 40 cells from three independent neuronal preparations. Numerical data are provided in Supplementary Data 1. Statistical significances, One-way ANOVA/Tukey’s posttest ( B–D ) and two-way ANOVA/Bonferroni′s test ( E , F ), respectively. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For exact p values see figure panels. Note that **** p < 0.0001 are too small values to be reported by the software used.

Article Snippet: For a schematic illustration of the dendritic evaluation parameters, see Supplementary Fig. . For examples of Imaris-based morphology tracings, see the 2D representations of 3D Imaris filaments with their branch and terminal points shown in Supplementary Figs. and .

Techniques: Staining, Transfection, Expressing, Marker, Software